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KMID : 0644120050180030032
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Journal of Oriental Gynecology
2005 Volume.18 No. 3 p.32 ~ p.50
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Effects of Saenghyeolbosu-Tang on the Morphometric Changes of Femur, and on the Factors Related with Bone Metabolism in Ovariectomized Rats
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Jeong Jae-Sook
Kim Hyung-Jun
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Abstract
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Purpose : This study was carried out to investigated the effects of Saenghyeolbosu-Tang on the morphometric changes of femur, and on the hormones and cytokines associated with bone metabolism in ovariectomized rats.
Methods : Twenty-four female Sprague-Dawley rats were divided into sham operated group(normal), ovariectomized group(control), and treated with extract of Saenghyeolbosu-Tang group(treated). Each group was evaluated the changes of body weight at 0, 3, 6, 8, weeks after ovariectomy. Morphometric analysis(femur weight, femur/body weight, femur ash weight, femur ash/body weight, cross sectional area of compact bone and cancellous bone of femur) and histopathological examination were performed at 8 weeks after ovariectomy. Estrogen and cytokines(TNF-{alpha},;IL-1{beta}, IL-6) assay were performed at 8 weeks after ovariectomy.
Results : 1. The femur weight(g) of treated group(1.59{pm}0.08) was significantly increased(p<0.05)compared with the control group(1.50{pm}0.07) at 8 weeks. 2. The femur/body weight(g) of treated group(5.27{pm}0.36) was significantly increased(p<0.05) compared with the control group(4.78{pm}0.37) at 8 weeks. 3. In the cross sectional area of cancellous bone of femur({mm^2}) the treated group(1.60{pm}0.20)was significantly increased(p<0.01) compared with the control group(1.19{pm}0.19) at 8 weeks. 4. The serum estrogen level(pg/ml) of treated(83.67{pm}27.44) and control group(800.49{pm}22.27)showed no significantly different changes at 8 weeks. 5. The serum TNF-{alpha} level(pg/ml) of treated(33.71{pm}6.43) and control group(41.35{pm}8.47) showed no significantly different changes at 8 weeks. 6. The serum IL-1{beta} level(pg/ml) of treated group(53.78{pm}10.83) was significantly decreased(p<0.001) compared with the control group(79.80{pm}11.40) at 8 weeks. 7. The serum IL-6 level(pg/ml) of treated group(55.52{pm}9.02) was significantly decreased(p<0.01) compared with the control group(69.69{pm}7.95) at 8 weeks.
Conclusion : This study shows that Saenghyeolbosu-Tang inhibits bone resolution in ovariectomized rats. These results may be related to the inhibitory effect of Saenghyeolbosu-Tang on the secretion of IL-1{\beta} and IL-6 in the Pathogenesis of osteoporosis.
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KEYWORD
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Saenghyeolbosu-Tang, osteoporosis, histopathology, IL-1{beta}, IL-6
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